Cat. No. GP62-C Synonyms sequestosome 1, ubiquitin-binding protein Suitable for IHC, WB / paraffin (microwave), frozen sections Presentation serum Host guinea pig Antibody Type polyclonal Dilution for IHC 1:100 Species / Reactive with human, mouse, rat Quantity 100 µL
C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.
Reactive Polypeptide (Specificity)
Human 62 kD (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy). The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer’s disease.
- immunohistochemistry on frozen and paraffin* sections (*enhanced after microwave treatment)
- cytological material - immunoblotting
1: 100 – 1:200 for immunohistochemistry
1:1,000 – 1:2000 for Western blotting (ECL)
1 h at RT; extended with paraffin sections
100 μl; contains 0.09% sodium azide